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resolving gel buffer for page  (Bio-Rad)
93
Bio-Rad resolving gel buffer for page
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Resolving Gel Buffer For Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0318prestained sds page standards  (Bio-Rad)
96
Bio-Rad 0318prestained sds page standards
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
0318prestained Sds Page Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0318prestained sds page standards - by Bioz Stars, 2026-06
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mini protean electrophoresis module assembly  (Bio-Rad)
96
Bio-Rad mini protean electrophoresis module assembly
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Mini Protean Electrophoresis Module Assembly, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean electrophoresis module assembly - by Bioz Stars, 2026-06
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native polyacrylamide gel  (Bio-Rad)
95
Bio-Rad native polyacrylamide gel
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Native Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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native polyacrylamide gel - by Bioz Stars, 2026-06
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polyacrylamide gel  (Bio-Rad)
93
Bio-Rad polyacrylamide gel
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
polyacrylamide gel - by Bioz Stars, 2026-06
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sds polyacrylamide gel electrophoresis  (Bio-Rad)
90
Bio-Rad sds polyacrylamide gel electrophoresis
a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing <t>gel</t> <t>electrophoresis</t> with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).
Sds Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds polyacrylamide gel electrophoresis - by Bioz Stars, 2026-06
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Image Search Results


a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Journal: Nature

Article Title: SIGLEC12 mediates plasma membrane rupture during necroptotic cell death

doi: 10.1038/s41586-025-09741-1

Figure Lengend Snippet: a – d , HT - 29 cells with the indicated knockouts ( a ) or knockdowns ( b – d ) were treated with TSE (30 ng ml −1 hTNF, 0.2 µM SM-164, 5 µM emricasan) or TRAIL + SE (50 ng ml −1 , 0.2 µM SM-164, 5 µM emricasan) for 8 h, and cell death or cell viability was quantified using the indicated assays ( P = 1.39 × 10 −13 ( a ), P < 1 × 10 −15 ( b ), P < 1 × 10 −15 ( c ) and P = 6.82 × 10 −12 , P = 1.23 × 10 −11 ( d )). Cell lysates were analysed using reducing or non-reducing gel electrophoresis with the indicated antibodies. e , HT-29 cells with indicated stable short hairpin RNA (shRNA)-mediated knockdowns were treated with TSE for the indicated times, and cell death was assessed using CellToxGreen and time-lapse fluorescence confocal microscopy. f , As in e , except cell morphology was visualized by scanning electron microscopy. g , As in e , except the release of HMGB1 was analysed by immunoblotting. Data are plotted as the mean ± s.d., representative of n = 4 ( c ), n = 9 ( a , d ) or n = 12 ( b ); three independent experiments. Statistical analyses were performed using two-tailed t -tests and two-way analyses of variance; **** P < 0.0001. Data are representative of three independent experiments ( d , middle and right, g ). Scale bars, 25 μm ( e ), 10 μm ( f ).

Article Snippet: The following reagents were used in this study: SM-164 (S7089), Nec-1s (S8641), GSK′872 (S8465), NSA (S8251), emricasan (S7775) and erastin (S7242) from SelleckChem; etoposide (E1383), α-ketoglutarate (349631), lipopolysaccharide (L4391), luminol (A8511), p -coumaric acid (C9008) and anti-FLAG M2 agarose beads (M8823) from Sigma-Aldrich; nigericin (11437) from the Cayman Chemical Company; Lipofectamine 3000 (L3000015), SuperSignal West Atto Ultimate Sensitivity Substrate (A38556) and Prolong Diamond Antifade Mountant with DAPI ( P36966 ) from Thermo Fisher Scientific; polyethylenimine (PEI; 24765-100) from Kyfora Bio; polybrene (TR-1003) from EMD Millipore; and 10X Tris/Glycine/SDS Electrophoresis Buffer (1610772), Tween 20 (1610781), Stacking Gel Buffer for PAGE (1610799), Resolving Gel Buffer for PAGE (1610798), Precision Plus Protein Dual Color Standards (1610394) and nitrocellulose membrane (1620115) from Bio-Rad.

Techniques: Nucleic Acid Electrophoresis, shRNA, Fluorescence, Confocal Microscopy, Electron Microscopy, Western Blot, Two Tailed Test